![]() ![]() Chances are you’ve probably gotten beyond the ΔΔCt method, but you should be prepared in case you face primer sets of different amplification efficiencies.īoth methods require the use of a housekeeping gene to control for differences in sample quantity, and both report the results as fold change of the target gene in test samples relative to control samples. ![]() As all of you probably know, methods for calculating relative gene expression from qPCR data include: a) double delta Ct (ΔΔCt) and b) that one other method.
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